Tendências e implicações do uso de inovações modernizantes no Brasil

Brazil; R&D; innovation

Fire performance of unprotected and protected concrete filled steel hollow structural sections

Concrete filled steel hollow structural (CFS) sections are increasingly used to support large compressive loads in buildings, with the concrete infill and the steel tube working together to yield several benefits both at ambient temperature and during a fire. These members are now widely applied in the design of highly optimized multi-storey and high rise buildings where fire resistance ratings of two or more hours may be required. Whilst the response and design of these sections at ambient temperatures is reasonably well understood, their response in fire, and thus their fire resistance design, is less well established. Structural fire resistance design guidance is available but has been developed based on tests of predominantly short, concentrically-loaded, small-diameter columns in braced frames using normal strength concrete. The current prescriptive guidance is limited and the design of CFS columns is thus often based on a detailed performance based approach, which can be time consuming and expensive and which is generally not well supported by a deep understanding of CFS columns’ behaviour in real fires. This thesis aims to understand the fundamental thermal and mechanical factors at play within these sections so as to provide guidance on how to improve their design for fire resistance when applied either as unprotected or protected sections. A meta-analysis of available furnace test data is used to demonstrate that current guidance fails to capture the relevant mechanics and thus poorly predicts fire resistance. It is also demonstrated that the predictive abilities of the available design standards vary with physical characteristics of the CFS section such as shape and size. A factor which has been observed in furnace tests on CFS sections but which is not accounted for in available guidance is the formation of an air gap between the steel tube and the concrete core due to differential expansion; this affects their structural response in fire. The insulating effect of air gap formation has not previously been addressed in literature and an experimental program is presented to systematically assess the effects of a gap on the heat transfer through the section; showing that the presence of even a 1 mm gap is important. To explicitly assess the heat transfer response within both unprotected and fire protected (i.e. insulated) CFS sections, 34 large scale standard furnace tests were performed in partnership with an industry sponsor. Fourteen tests on large scale unloaded unprotected CFS sections are presented to assess current capability to predict the thermal response and to assess the effects of different sectional and material parameters on heating. New best practice thermal modelling guidance is suggested based on comparison between the models and observed temperatures from the tests. Twenty CFS specimens of varying size and shape, protected with different types and thicknesses of intumescent paint fire insulation, were also tested unloaded in a furnace to understand the thermal evolution within protected CFS sections and to develop design guidance to support application of intumescent coatings in performance based fire resistance design of CFS sections. These tests demonstrate that the intumescent coatings were far more effective than expected when applied to CFS sections, and that current methods of designing the coatings’ thickness are overly conservative. The reason for this appears to be that the calculation of effective section factor which is used in the prescription of intumescent coating thicknesses is based on the thermal response of unprotected CFS sections which display fundamentally different heating characteristics from protected sections due to the development of a thermal gradient in the concrete core. It is also demonstrated (by calculation supported by the testing presented herein) that the steel failure temperature (i.e. limiting temperature) of an unprotected CFS column in fire is significantly higher than one which is protected; procedures to determine the limiting temperature of protected sections are suggested. Finally, the residual strength of fire-exposed CFS columns is examined through structural testing of 19 of the 34 fire tested columns along with unheated control specimens. The results provide insights into the residual response of unprotected and protected CFS section exposed to fire, and demonstrate a reasonable ability to calculate their residual structural capacity. The work presented in this thesis has shed light on the ability of available guidance to rationally predict the thermal and structural response to fire of CFS columns, has improved the understanding of the thermal evolution within protected and unprotected CFS sections in fire, has provided best-practice guidance and material input parameters for both thermal and structural modelling of CFS sections, and has improved understanding of the residual capacity of CFS sections after a fire

Efficacy of the Mnemonic Device “MARCH PAWS” as a Checklist for Pararescuemen during Tactical Field Care and Tactical Evacuation

Background: The application of TCCC represents evidence-based medicine to improve survival in combat. Over the past several years, USAF Pararescuemen (PJs) have expanded the mnemonic device “MARCH” to “MARCH PAWS” for use during tactical field care and tactical evacuation. It stands for massive bleeding, airway, respiration, circulation, head and hypothermia, pain, antibiotics, wounds, and splinting. We undertook this performance improvement project to determine the efficacy of this device as a treatment checklist. Methods: The mission reports of a sixteen PJs’ combat rescue deployment to OEF from January through June 2012 were reviewed. The triage category, mechanism of injury, injury, and treatments were noted. The treatments were then categorized to determine if they were included in “MARCH PAWS”. Results: The recorded data for missions involving four hundred sixty-five patients comprise the study. 45%, 48% and 7 %, were in Category A, B and C respectively (urgent, priority, routine). 55% were battle injuries (BI) and 45% were non-battle injuries (NBI). All treatments for BI were accounted for in MARCH PAWS. Only 9 patients’ treatments with NBI were not in MARCH PAWS. Conclusion: This simple mnemonic device is a reliable checklist for PJs, corpsmen, and medics to perform tactical field care and tactical evacuation during combat operations, as well as care for non-combat trauma patients

In situ characterization of CD4+ T cell behavior in mucosal and systemic lymphoid tissues during the induction of oral priming and tolerance

The behavior of antigen-specific CD4+ T lymphocytes during initial exposure to antigen probably influences their decision to become primed or tolerized, but this has not been examined directly in vivo. We have therefore tracked such cells in real time, in situ during the induction of oral priming versus oral tolerance. There were marked contrasts with respect to rate and type of movement and clustering between naive T cells and those exposed to antigen in immunogenic or tolerogenic forms. However, the major difference when comparing tolerized and primed T cells was that the latter formed larger and longer-lived clusters within mucosal and peripheral lymph nodes. This is the first comparison of the behavior of antigen-specific CD4+ T cells in situ in mucosal and systemic lymphoid tissues during the induction of priming versus tolerance in a physiologically relevant model in vivo

Falkland Island peatland development processes and the pervasive presence of fire

Acknowledgments RJP secured funding for this research from the Quaternary Research Association, University of York and the Russian Science Foundation (19-14-00102). We thank Paul Brickle and other members of the South Atlantic Environmental Research Institute for their help with logistics, David Large for valuable discussions about Falkland Islands peat and all landowners for access permission. This work is dedicated to Richard J. Payne who was tragically killed while climbing Peak 6477, a previously unclimbed subsidiary peak of Nanda Devi (Garhwal Himalayas) in May 2019. CRediT authorship contribution statement Dmitri Mauquoy: Conceptualization, Investigation, Writing - original draft, Writing - review & editing. Richard J. Payne: Conceptualization, Investigation. Kirill V. Babeshko: Investigation, Writing - original draft, Writing - review & editing. Rebecca Bartlett: Investigation, Writing - original draft, Writing - review & editing. Ian Boomer: Investigation. Hannah Bowey: Investigation. Chris D. Evans: Conceptualization, Writing - original draft, Writing - review & editing. Fin Ring-Hrubesh: Investigation. David Muirhead: Methodology, Investigation, Writing - original draft, Writing - review & editing. Matthew O’Callaghan: Investigation. Natalia Piotrowska: Investigation. Graham Rush: Investigation. Thomas Sloan: Investigation. Craig Smeaton: Methodology, Investigation, Writing - original draft. Andrey N. Tsyganov: Investigation, Writing - original draft, Writing - review & editing. Yuri A. Mazei: Investigation, Writing - original draft, Writing - review & editing.Peer reviewedPostprin

HLA-DR/DQ molecular mismatch: A prognostic biomarker for primary alloimmunity.

Alloimmune risk stratification in renal transplantation has lacked the necessary prognostic biomarkers to personalize recipient care or optimize clinical trials. HLA molecular mismatch improves precision compared to traditional antigen mismatch but has not been studied in detail at the individual molecule level. This study evaluated 664 renal transplant recipients and correlated HLA-DR/DQ single molecule eplet mismatch with serologic, histologic, and clinical outcomes. Compared to traditional HLA-DR/DQ whole antigen mismatch, HLA-DR/DQ single molecule eplet mismatch improved the correlation with de novo donor-specific antibody development (area under the curve 0.54 vs 0.84) and allowed recipients to be stratified into low, intermediate, and high alloimmune risk categories. These risk categories were significantly correlated with primary alloimmune events including Banff ≥1A T cell-mediated rejection (P = .0006), HLA-DR/DQ de novo donor-specific antibody development (P < .0001), antibody-mediated rejection (P < .0001), as well as all-cause graft loss (P = .0012) and each of these correlations persisted in multivariate models. Thus, HLA-DR/DQ single molecule eplet mismatch may represent a precise, reproducible, and widely available prognostic biomarker that can be applied to tailor immunosuppression or design clinical trials based on individual patient risk

Antigen-Engaged B Cells Undergo Chemotaxis toward the T Zone and Form Motile Conjugates with Helper T Cells

Interactions between B and T cells are essential for most antibody responses, but the dynamics of these interactions are poorly understood. By two-photon microscopy of intact lymph nodes, we show that upon exposure to antigen, B cells migrate with directional preference toward the B-zone–T-zone boundary in a CCR7-dependent manner, through a region that exhibits a CCR7-ligand gradient. Initially the B cells show reduced motility, but after 1 d, motility is increased to approximately 9 μm/min. Antigen-engaged B cells pair with antigen-specific helper T cells for 10 to more than 60 min, whereas non-antigen-specific interactions last less than 10 min. B cell–T cell conjugates are highly dynamic and migrate extensively, being led by B cells. B cells occasionally contact more than one T cell, whereas T cells are strictly monogamous in their interactions. These findings provide evidence of lymphocyte chemotaxis in vivo, and they begin to define the spatiotemporal cellular dynamics associated with T cell–dependent antibody responses

Using participatory design methodologies to co-design and culturally adapt the Spanish version of the Mental Health eClinic: Qualitative study

Background: The Mental Health eClinic (MHeC) aims to deliver best-practice clinical services to young people experiencing mental health problems by making clinical care accessible, affordable, and available to young people whenever and wherever they need it most. The original MHeC consists of home page with a visible triage system for those requiring urgent help; a online physical and mental health self-report assessment; a results dashboard; a booking and videoconferencing system; and the generation of a personalized well-being plan. Populations who do not speak English and reside in English-speaking countries are less likely to receive mental health care. In Australia, international students have been identified as disadvantaged compared with their peers; have weaker social support networks; and have higher rates of psychological distress. This scenario is acquiring significant relevance as Spanish-speaking migration is rapidly growing in Australia, and the mental health services for culturally and linguistically diverse populations are limited. Having a Spanish version (MHeC-S) of the Mental Health eClinic would greatly benefit these students. Objective: We used participatory design methodologies with users (young people aged 16-30 years, supportive others, and health professionals) to (1) conduct workshops with users to co-design and culturally adapt the MHeC; (2) inform the development of the MHeC-S alpha prototype; (3) test the usability of the MHeC-S alpha prototype; (4) translate, culturally adapt, and face-validate the MHeC-S self-report assessment; and (5) collect information to inform its beta prototype. Methods: A research and development cycle included several participatory design phases: co-design workshops; knowledge translation; language translation and cultural adaptation; and rapid prototyping and user testing of the MHeC-S alpha prototype. Results: We held 2 co-design workshops with 17 users (10 young people, 7 health professionals). A total of 15 participated in the one-on-one user testing sessions (7 young people, 5 health professionals, 3 supportive others). We collected 225 source documents, and thematic analysis resulted in 5 main themes (help-seeking barriers, technology platform, functionality, content, and user interface). A random sample of 106 source documents analyzed by 2 independent raters revealed almost perfect agreement for functionality (kappa=.86; P\u3c.001) and content (kappa=.92; P\u3c.001) and substantial agreement for the user interface (kappa=.785; P\u3c.001). In this random sample, no annotations were coded for help-seeking barriers or the technology platform. Language was identified as the main barrier to getting medical or psychological services, and smartphones were the most-used device to access the internet. Acceptability was adequate for the prototype’s 5 main elements: home page and triage system, self-report assessment, dashboard of results, booking and video visit system, and personalized well-being plan. The data also revealed gaps in the alpha prototype, such as the need for tailored assessment tools and a greater integration with Spanish-speaking services and communities. Spanish-language apps and e-tools, as well as online mental health information, were lacking. Conclusions: Through a research and development process, we co-designed and culturally adapted, developed and user tested, and evaluated the MHeC-S. By translating and culturally adapting the MHeC to Spanish, we aimed to increase accessibility and availability of e-mental health care in the developing world, and assist vulnerable populations that have migrated to English-speaking countries

Mouse Protocadherin-1 gene expression is regulated by cigarette smoke exposure in vivo

Protocadherin-1 (PCDH1) is a novel susceptibility gene for airway hyperresponsiveness, first identified in families exposed to cigarette smoke and is expressed in bronchial epithelial cells. Here, we asked how mouse Pcdh1 expression is regulated in lung structural cells in vivo under physiological conditions, and in both short-term cigarette smoke exposure models characterized by airway inflammation and hyperresponsiveness and chronic cigarette smoke exposure models. Pcdh1 gene-structure was investigated by Rapid Amplification of cDNA Ends. Pcdh1 mRNA and protein expression was investigated by qRT-PCR, western blotting using isoform-specific antibodies. We observed 87% conservation of the Pcdh1 nucleotide sequence, and 96% conservation of the Pcdh1 protein sequence between men and mice. We identified a novel Pcdh1 isoform encoding only the intracellular signalling motifs. Cigarette smoke exposure for 4 consecutive days markedly reduced Pcdh1 mRNA expression in lung tissue (3 to 4-fold), while neutrophilia and airway hyperresponsiveness was induced. Moreover, Pcdh1 mRNA expression in lung tissue was reduced already 6 hours after an acute cigarette-smoke exposure in mice. Chronic exposure to cigarette smoke induced loss of Pcdh1 protein in lung tissue after 2 months, while Pcdh1 protein levels were no longer reduced after 9 months of cigarette smoke exposure. We conclude that Pcdh1 is highly homologous to human PCDH1, encodes two transmembrane proteins and one intracellular protein, and is regulated by cigarette smoke exposure in vivo